Figure S1.

Ptpn2 regulates early epidermal infiltration of OT-I cells independently of local antigen. (A) In vitro activated Ptpn2^fl/fl and Lck-Cre;Ptpn2^fl/fl OT-I cells were cultured with IL-2 (25 U/ml) in the presence or absence (Ctrl) of TGFβ (10 ng/ml), as indicated. Analysis of CD103 expression 2 d after addition of TGFβ; data are representative of n = 2 experiments. (B) Enumeration of CD103⁺ Ptpn2^fl/fl (white) or Lck-Cre;Ptpn2^fl/fl (orange) OT-I cells in epidermis and dermis in HSV-Ova (left flank)– and WT HSV (right flank)–infected skin 9 d after infection. Data were pooled from n = 2 experiments with n = 7 mice/group. (C and D) Means of KLRG1⁻ OT-I cell numbers in spleen (C) and skin (D) from individual experiments 9 d after single or double HSV infection as in Fig. 1 H; data points from the same individual experiments (n = 7) connected by lines. Statistical significance (*, P < 0.05) determined by Wilcoxon test. (E) In vitro activated gBT-I cells transduced with pMIG.Klrg1.WT (left, middle) or pMIG.Ctrl (right) were cultured with IL-2 (25 U/ml) in the presence or absence (Ctrl) of TGFβ (10 ng/ml), as indicated. Analysis of KLRG1 and CD103 expression by GFP⁺ gBT-I cells 3 d after addition of TGFβ; data are representative of n = 2 experiments.