Figure S5.

Ptpn2 regulates T_RM effector functions. (A) Representative photos of ipsilateral (left) and contralateral (right) flanks of K5-rTA;TetO.mOva mice 5 d after start of Dox treatment (1 mg/ml in drinking water). Mice received in vitro–activated Ptpn2^fl/fl (top) and Lck-Cre;Ptpn2^fl/fl (bottom) OT-I cells (5 × 10⁶) by i.d. transfer >4 wk prior, as in Fig. 5 B. Photos depict whole flanks of mice. (B) Mean fluorescence intensity (MFI) for granzyme B–expressing Ptpn2^fl/fl (black) and Lck-Cre;Ptpn2^fl/fl (orange) T_RM cells in K5-rTA;TetO.mOva mice 5 d after start of Dox treatment (Fig. 5 D). Data pooled from n = 2 experiments with n = 7 mice per group. (C) Analysis of granzyme B and IFNɣ expression by Ptpn2^fl/fl (white) and Lck-Cre;Ptpn2^fl/fl (orange) T_RM cells 6 h after i.d. injection of Ova peptide (SIINFEKL, 0.1 µg). Mice had received in vitro–activated OT-I cells (2 × 10⁶) by i.d. transfer 4 wk prior, as in Fig. 4 C. n = 6 mice per group from one experiment. Statistical significance (**, P < 0.01) determined by Mann–Whitney test.