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Relative uptake of 13 by investigating a) dose concentration and b) mechanism of uptake (all treatments lasted 4 h). For Student’s t-test analysis, p < 0.05 (*) or p < 0.001 (***). Error bars represent one standard deviation from three trials. CPZ represents 25 μM chlorpromazine. For the four total 5 μM and 15 μM treatments in b), propidium iodide was used to gate only live cells because dead cells may have different uptake, and chlorpromazine was somewhat toxic to the cells.
