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. 2021 Apr 21;6(11):4083–4095. doi: 10.1016/j.bioactmat.2021.01.013

Fig. 6.

Fig. 6

In vitro osteogenic differentiation assays of BOPSCs and control scaffolds. a) Microenvironment schematic of in-situ osteogenic induction of mADSCs in interior pore of BOPSCs with BMP2-release. b) CLSM observation of OCN expression and distribution of mADSCs in BOPSCs and control scaffolds at day 15. c) ALP activity assay of mADSCs cultured on BOPSCs and control scaffolds. ALP activity was determined as enzyme activity units (U) per milligram of protein. d-h), Quantitative PCR analysis of osteogenic gene marker expression in mADSCs cultured on BOPSCs and control scaffolds after 7 and 14 days, respectively, including d) runt-related transcription factor 2 (Runx2), e) osteopontin (OPN), f) collagen-type I (COL-1), g) osteocalcin (OCN), and h) bone sialoprotein (BSP). The Y-axis represents the relative expression (2−ΔCT) normalized to the expression level of the housekeeping gene GAPDH. Statistically significant difference in c)-h), ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, n.s. = no significant difference, n = 3.