Figure 1.

Cell-poor tissue is sensitive to repetitive high-temperature heating, while morphology is well conserved after multiple treatments with stripping buffer. On top is shown, hematoxylin and eosin staining of human skin (A), lung (B), and tonsil (C) tissue before HIER. At the bottom is shown treatment of skin (A), lung (B), and tonsil (C) tissue after one treatment (left) and five subsequent treatments (right) with HIER in citrate buffer, HIER in Tris-EDTA buffer, or β-mercaptoethanol-containing stripping buffer. Bars equal 1 mm. Abbreviation: HIER, heat-induced epitope retrieval; EDTA, ethylenediaminetetraacetic acid; βME, β-mercaptoethanol.