Figure 2.

Sensitivity of epitopes for repetitive treatment with stripping buffer. (A) CD3-stained human skin tissue (left) was subjected to one round of treatment with β-mercaptoethanol-containing stripping buffer followed by incubation with poly-HRP anti-rabbit and NovaRED visualization (right). Lack of staining after treatment with staining buffer indicated successful removal of primary and secondary antibodies. (B) Melan-A staining on human skin tissue after one round of HIER treatment with Tris-EDTA buffer, followed by indicated rounds of treatment with stripping buffer. Antibody dilutions are indicated. Bars equal 100 µm. Abbreviation: HIER, heat-induced epitope retrieval; EDTA, ethylenediaminetetraacetic acid.