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. 2020 Oct 29;31(4):391–409. doi: 10.1093/glycob/cwaa100

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© The Author(s) 2020. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Reversed phase HPLC profiles of the urinary acidic free-glycans. PA-labeled free glycans fractionated by normal phase HPLC were further separated by reversed phase HPLC on a TSK-gel ODS-80Ts column. The amount of urine sample corresponded to 16 μg of creatinine. Representative overlaid chromatograms are shown from six normal controls (N5–N8, N11 and N14), black line; three gastric cancer patients (G8, G11 and G12), blue dotted line; two pancreatic cancer patients (P3 and P9) and one cholangiocarcinoma patient (C2), red dotted line. The major glycans comprising the fluorescent peaks are indicated as numbers. Estimated structures of the numbered glycans are shown in Table II in accordance with their basal structure and in supplementary data (Supplementary Table SII) in numerical order. X indicate peaks mainly composed of non-PA-glycan fluorescent materials. (A) The chromatograms of Fr 1–4, which contained more abundant glycans, are shown at a lower magnification of ×0.1. (B) The chromatograms of Fr 1–Fr 12.

Fig. 3 — Reversed phase HPLC profiles of the urinary acidic free-glycans. PA-labeled free glycans fractionated by normal phase HPLC were further separated by reversed phase HPLC on a TSK-gel ODS-80Ts column. The amount of urine sample corresponded to 16 μg of creatinine. Representative overlaid chromatograms are shown from six normal controls (N5–N8, N11 and N14), black line; three gastric cancer patients (G8, G11 and G12), blue dotted line; two pancreatic cancer patients (P3 and P9) and one cholangiocarcinoma patient (C2), red dotted line. The major glycans comprising the fluorescent peaks are indicated as numbers. Estimated structures of the numbered glycans are shown in Table II in accordance with their basal structure and in supplementary data (Supplementary Table SII) in numerical order. X indicate peaks mainly composed of non-PA-glycan fluorescent materials. (A) The chromatograms of Fr 1–4, which contained more abundant glycans, are shown at a lower magnification of ×0.1. (B) The chromatograms of Fr 1–Fr 12.