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1H–13C HSQCs of acetyl methyl region of CEACAM1-IgV. (A) Supplemented with u-13C-glucose and grown in HEK293S (GnTI-) cells leading to a dominant Man5GlcNAc2 glycan population. (B) Similarly prepared CEACAM1-IgV with an additional Endo-F treatment to trim the glycans to a single GlcNAc. (C) Analogous to A, but a CEACAM1-IgV S96C mutant collected with a constant time HSQC. (D) Projections of a slice of A (−o-) and B (−), normalized to the noise.
