Table 1.
Strains and plasmids used in this study.
| Name | Description | Reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | deoR recA1 endA1 hsdR17(rk− mk+) phoA supE44 λ−thi-1 gyrA96 relA1 | Takara |
| E. coli DH1 | supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Our laboratory |
| E. coli DMT | lacZΔM15Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk− mk+) phoA supE44 thi-1 gyrA96 relA1 tonA | TRANSGEN |
| Strains used for lycopene production | ||
| DH411 | DH06 Δ23th:: T7lac GC (counter-clockwise) Δ64th:: T7 S1 Δ57th:: T7 ES araB::T7RNAP-tetA | [49] |
| DH412 | DH411 Δ8th:: T7 GC (counter-clockwise) | This study |
| DH422 | DH411 Δ44th:: T7 GC | This study |
| DH432 | DH411 Δ58th:: T7 GC | This study |
| DH442 | DH411 ΔlpxM:: T7 GC (counter-clockwise) | This study |
| DH414 | DH412 ΔlpxM:: T7 GC (counter-clockwise) | This study |
| DH416 | DH414 Δ58th:: T7 S1 | This study |
| D411E | DH411/pCC1E | This study |
| D411S | DH411/pCC1S | This study |
| D411G | DH411/pCC1G | This study |
| D411ES | DH411/pCC1ES | This study |
| D411EG | DH411/pCC1EG | This study |
| D411SG | DH411/pCC1SG | This study |
| D412SS | DH412/pCC1SS | This study |
| D412SG | DH412/pCC1SG | This study |
| D422SG | DH422/pCC1SG | This study |
| D432SG | DH432/pCC1SG | This study |
| D442SG | DH442/pCC1SG | This study |
| D414S | DH414/pCC1S | This study |
| D414SS | DH414/pCC1SS | This study |
| D414SG | DH414/pCC1SG | This study |
| Plasmids | ||
| pET3b | pMB1 origin, T7 promoter; AmpR | Novagen |
| Pete | pET3b derived, carrying T7 ES; AmpR | Our laboratory |
| pETS | pET3b derived, carrying T7 S1; AmpR | [49] |
| pETG | pET3b derived, carrying T7 GC; AmpR | [49] |
| pCC1FOS | oriV, ori2, repE, parA, parB, parC, cos, loxP, lacZ, redF; CmR | Epicentre |
| pCC1E | pCC1FOS derived, carrying T7 ES; CmR | This study |
| pCC1S | pCC1FOS derived, carrying T7 S1; CmR | [49] |
| pCC1G | pCC1FOS derived, carrying T7 GC; CmR | This study |
| pCC1ES | pCC1FOS derived, carrying T7 ES and T7 S1; CmR | This study |
| pCC1EG | pCC1FOS derived, carrying T7 ES and T7 GC; CmR | This study |
| pCC1SS | pCC1FOS derived, carrying T7 S1 and T7 S1; CmR | [49] |
| pCC1SG | pCC1FOS derived, carrying T7 S1 and T7 GC; CmR | [49] |
| Plasmid used for integration | ||
| Helper plasmid | ||
| pCNA | pKOBEG derived, carrying I-CreI endonuclease gene at Nhe I site; AmpR | [52] |
| pSNA | pKOBEG derived, carrying I-SceI endonuclease gene at Nhe I site; AmpR | [52] |
| pSNK | pKOBEG derived, carrying I-SceI endonuclease gene at Nhe I site; KanR | [52] |
| Landing pad plasmid | ||
| pBDC | p15A origin, sacB cassette, I-CreI sites; CmR | [58] |
| pBDK | p15A origin, sacB cassette, I-CreI sites; KanR | [58] |
| pBDC-8ri | pBDC derived, carrying eighth homologous region; CmR | [52] |
| pBDC-44i | pBDC derived, carrying 44th homologous region; CmR | [52] |
| pBDC-58i | pBDC derived, carrying 58th homologous region; CmR | [52] |
| pBDC-Li | pBDC derived, carrying lpxM homologous region; CmR | [49] |
| Donor plasmid | ||
| pKI | pMB1 origin, I-SceI sites; KanR | [49] |
| pETIS | pET3b derived, I-SceI sites; AmpR | [49] |
| pKILE | pMB1 origin, T7 ES, I-SceI sites; KanR, carrying lpxM homologous region | [49] |
| pKIS | pKIS derived, carrying T7 S1; KanR | [49] |
| pETISG | pETIS derived, carrying T7 GC; AmpR | [49] |
| pKLIG | pMB1 origin, T7 GC, I-SceI sites; KanR, carrying lpxM homologous region | This study |