Table 2.

Editing results obtained by amplicon sequencing (replicates 1 and 2)

Sample name	Replicate ID	Tot reads	Reads “T” allele	%AF T	Reads “C” allele	%AF C	Reads del	%AF del	Reads ins	%AF ins
Th-ErPC-2 C−	1	458,006	456,607	99.7	725	0.2	19	0	0	0
	1A	218,508	217,278	99.4	318	0.1	6	0	0	0
Th-ErPC-2 GE Fwd	2	340,907	24,731	7.3	38,805	11.4	53,527	15.7	622	0.2
	2A	228,217	162,305	71.1	24,163	10.6	40,733	17.8	464	0.2
Th-ErPC-2 GE Rev	3	487,416	354,441	72.7	69,534	14.3	61,375	12.6	444	0.1
	3A	291,744	210,678	72.2	41,252	14.1	3,861	13.2	203	0.1
Th-ErPC-4 C−	4	427,363	425,828	99.6	715	0.2	32	0.01	0	0
	4A	291,416	290,403	99.7	468	0.2	19	0.01	1	0
Th-ErPC-4 GE Fwd	5	417,987	354,157	84.7	28,731	6.9	33,668	8.1	401	0.1
	5A	270,785	230,672	85.2	17,534	6.5	21,653	8.0	241	0.1
Th-ErPC-4 GE Rev	6	397,882	335,472	84.3	29,596	7.4	31,592	7.9	53	0
	6A	276,418	233,346	84.4	20,950	7.6	21,124	7.6	52	0
Th-ErPC-6 C−	7	461,288	459,674	99.7	893	0.2	23	0	0	0
	7A	262,450	261,364	99.6	557	0.2	53	0.02	0	0
Th-ErPC-6 GE Fwd	8	377,829	31,396	8.3	26,798	7.1	35,671	9.4	538	0.1
	8A	262,572	218,433	83.2	23,086	8.8	19,472	7.4	1,206	0.5
Th-ErPC-6 GE Rev	9	428,089	326,031	76.2	61,707	14.4	38,718	9.0	576	0.1
	9A	364,864	2,9097	80	35,703	9.8	36,575	10.0	589	0.2

The columns report the sample name, the replicate identification (ID), the total (Tot) number of reads containing the base position (chr11:5,226,774, corresponding to the β⁰39 mutation), the number of reads containing the allele T (mutated), the allele frequency (AF) of T, the number of reads containing the allele C (not mutated), the AF of C, the number of reads containing the deletion (del), the AF of del, the number of reads containing the insertion (ins), and the AF of ins.