Table 3.

List of oligonucleotides used to conduct the different analyses and evaluate the correction degree obtained on the β-globin gene and mRNA with the CRISPR-Cas9 system

Oligonucleotide and assay	Sequence	Application
α-globin forward primer	5′-GGTCTTGGTGGTGGGGAAG-3′	qRT-PCR
α-globin reverse primer	5′-CGACAAGACCAACGTCAAGG-3′	qRT-PCR
α-globin probe	5′-/5HEX/ACATCCTCT/ZEN/CCAGGGCCTCCG/3IABkFQ/-3′	qRT-PCR
β-globin forward primer	5′-GGTGAATTCTTTGCCAAAGTGAT-3′	qRT-PCR
β-globin reverse primer	5′-GGGCACCTTTGCCACAC-3′	qRT-PCR
β-globin probe	5′-/5Cy5/ACGTTGCCCAGGAGCCTGAAG/3IAbRQSp/-3′	qRT-PCR
γ-globin forward primer	5′-TTCTTTGCCGAAATGGATTGC-3′	qRT-PCR
γ-globin reverse primer	5′-TGACAAGCTGCATGTGGATC-3′	qRT-PCR
γ-globin probe	5′-/56-FAM/TCACCAGCA/ZEN/CATTTCCCAGGAGC/3IABkFQ/-3′	qRT-PCR
GAPDH forward primer	5′-TGTAGTTGAGGTCAATGAAGGG-3′	qRT-PCR
GAPDH reverse primer	5′-ACATCGCTCAGACACCATG-3′	qRT-PCR
GAPDH probe	5′-/56-FAM/AAGGTCGGTCGGA/ZEN/GTCAACGGATTTGGTC/3IABkFQ/-3′	qRT-PCR
β-actin forward primer	5′-ACAGAGCCTCGCCTTTG-3′	qRT-PCR
β-actin reverse primer	5′-ACGATGGAGGGGAAGACG-3′	qRT-PCR
β-actin probe	5′-/5Cy5/CCTTGCACATGCCGGAGCC/3IAbRQSp/-3′	qRT-PCR
β-globin forward primer	5′-CACTGACTCTCTCTGCCTATTG-3′	ddPCR β-globin gene
β-globin reverse primer	5′-ACCTTAGGGTTGCCCATAAC-3′	ddPCR β-globin gene
β-globin β039 probe (HEX)	5′-/5HEX/TCTACCCTT/ZEN/GGACCTAGAGGTTCT/3IABkFQ/-3′	ddPCR β-globin gene
β-globin edit probe (FAM)	5′-/56-FAM/TCTACCCTT/ZEN/GGACCCAGAGATTCT/3IABkFQ/-3′	ddPCR β-globin gene
β-globin forward primer	5′-TGGATGAAGTTGGTGGTGAG-3′	ddPCR β-globin mRNA
β-globin reverse primer	5′-CCTTAGGGTTGCCCATAACA-3′	ddPCR β-globin mRNA
β-globin β039 probe (HEX)	5′-/5HEX/TCTACCCTT/ZEN/GGACCTAGAGGTTCTT/3IABkFQ/-3′	ddPCR β-globin mRNA
β-globin edit probe (FAM)	5′-/56-FAM/TCTACCCTT/ZEN/GGACCCAGAGGTTCTT/3IABkFQ/-3′	ddPCR β-globin mRNA
BGF β-globin forward primer	5′-GTGCCAGAAGAGCCAAGGACAGG-3′	β-globin PCR and sequencing
T12R β-globin reverse primer	5′-AGTTCTCAGGATCCACGTGCA-3′	β-globin PCR and sequencing