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. 2021 Apr 19;12:639256. doi: 10.3389/fphar.2021.639256

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Copyright © 2021 Yang, Li, Xie, Wang, Wen, Zhao, Tao, Li, Tian and Sheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Astragalin inhibited the migration of HCT116 cells with effects on migration and invasion. (A,B) Colony experiment showing effects of astragalin on HCT116 cells. (C,D) Wound healing assay in HCT116 cells showing inhibition by astragalin (scratch analysis). (E,F) Transwell invasion assay showing that astragalin inhibited migration of HCT116 cells. (G,H) Astragalin inhibited migration of HCT116 cells by transwell migrate assay. (I,J) expression levels of MMP-9 and MMP-2 as determined by Elisa. Data are expressed as mean ± SEM from three independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. 0.0 μg/ml.