FIGURE 4.

Astragalin inhibited NF-κB signaling pathways. (A,B) Protein expression of Iκκα, p-Iκκα, IκBα, p-IκBα, NF-κB, p-NF-κB, NF-κB P65 determined by western blot after cells were exposed to astragalin for 48 h. (C,D) Protein expression of p-NF-κB and P-NF-κB P65 determined by western blot after cells were exposed to astragalin for a short time treatment. (E,F) expression levels of TNF-α and IL-6 as determined by Elisa. Data are expressed as mean ± SEM from three independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. 0.0 μg/ml.