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. 2021 Apr 19;12:639256. doi: 10.3389/fphar.2021.639256

FIGURE 5.

FIGURE 5

Astragalin inhibited NF-κB signaling pathways in HCT116 cells stimulated with TNF-α. (A,B) Protein expression of IκBα, p-IκBα, NF-κB, p-NF-κB, NF-κB P65, P-NF-κB P65 determined by western blot after cells were exposed to astragalin and TNF-α. (C,D) Protein expression of NF-κB P65 in the cytosol and nuclear determined by western blot after cells were stimulated with TNF-α. (E,F) Immunofluorescence confocal staining for determining NF-κB P65 nuclear translocation. Data are expressed as mean ± SEM from three independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001. vs. Control, # p < 0.05, ## p < 0.01, ### p < 0.001 vs. TNF-α group.