Figure 4.

Immunofluorescence staining of NSCs and GSCs in the SVZ of glioblastoma patients. CD133 and CD9 expression in the SVZ of glioblastoma patients (A–D) with active ECs (A, C) and quiescent ECs (B) with variable CD133 expression (medium in A, high in B, and low in C). NSCs are characterized by CD133-positive CD9-negative and GSCs by CD133-positive CD9-positive. D is a magnification of section in C indicated by a yellow rectangle. DAPI is used for nuclear counterstaining. Scale bars: A–C = 100 µm; D = 50 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; EC, ependymal cell; LV, lateral ventricle; NSC, neural stem cell. SVZ, subventricular zone.