Table 1.

Details of Primary Antibodies Used for Fluorescence Immunohistochemistry.

Primary Antibody	Source	Primary Antibody Dilution in PBS + 1% BSA for Fluorescence IHC	Protein Function/Characteristics
Rabbit anti-human CD133	Abcama (ab19898)	1:100	Biomarker to detect NSCs and GSCs in glioblastoma
Mouse anti-human CD9	Abcam (ab2215)	1:100	Biomarker to detect GSCs in glioblastoma
Mouse anti-human SOX2	Abcam (ab171380)	1:50	Transcription factor expressed in GSCs in glioblastoma
Mouse anti-human GFAP	Abcam (ab10062)	1:500	Intermediate filament protein expressed in astrocytes
Rabbit anti-human SDF-1α	Abcam (ab9797)	1:200	Chemokine for CXCR4-positive (stem) cells
Rabbit anti-human CXCR4	R&D Systemsb clone 44716 (MAb172)	1:30	G-protein-coupled receptor that interacts with ligand SDF-1α
Rabbit anti-human OPN	Abcam (ab8448)	1:100	Chemokine for CD44-positive cells
Mouse anti-human CD44	Bioradc (MCA2504)	1:100	Receptor that interacts with ligand OPN
Mouse anti-human HIF-1α	Abcam (ab8366)	1:100	Transcription factor expressed in cells in hypoxic conditions
Rabbit anti-human HIF-2α	Abcam (ab109616)	1:100	Transcription factor expressed in cells in hypoxic conditions

Abbreviations: PBS, phosphate-buffered saline; BSA, bovine serum albumin; IHC, immunohistochemistry; GSC, glioblastoma stem cell; GFAP, glial fibrillary acidic protein; SOX2, sex-determining region Y-box 2; SDF-1α, stromal-derived factor-1α; CXCR4, C-X-C receptor type 4; OPN, osteopontin; HIF, hypoxia-induced factor.

^aAbcam, Cambridge, UK.

^bR&D Systems, Minneapolis, MN, USA.

^cBiorad, Hercules, CA, USA.