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. 2021 May 3;12:60. doi: 10.1186/s40104-021-00582-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — The expression of six differentially expressed (DE) lncRNAs (a), six DE miRNAs (c) and seven DE genes (e) validated with qRT-PCR in chicken embryo chorioallantoic membrane (CAM) tissue. Note: The Y-axis represents the expression value of RNA in chicken embryo CAM, and the X-axis represents the types of RNAs. Error bars represent SE of expression. * on the bars indicate P < 0.05 and ** indicate P < 0.01 between TC and CH breeds. Tibetan chickens (TCs, n = 4–8), and Chahua chickens (CHs, n = 4–8). The fold changes of the six DE lncRNAs (b), six DE miRNAs (d) and seven DE genes (f) showed that qRT-PCR results were consistent with the RNA-seq data