Table 1.
Potential key differentially expressed mRNAs (DE genes), their targeted differentially expressed miRNAs (DE miRNAs)/differentially expressed lncRNAs (DE lncRNAs), and their functions related to hypoxic adaption in the Tibetan chicken
| DE genes | Log2FC (TC/CH) | P-value | Targeted DE miRNAs | Targeted DE lncRNAs | Functional description |
|---|---|---|---|---|---|
| Angiogenesis | |||||
| ACTC1 | −3.4644 | 0.0122 | gga-miR-6606-5p | MSTRG.19949.13, MSTRG.128588.3, MSTRG.73129.28, MSTRG.32082.2 | Blood circulation |
| KCNMB4 | 2.3558 | 0.0001 | gga-miR-6606-5p | MSTRG.29252.7 | Vascular smooth muscle contraction |
| NCS1 | −1.0050 | 0.0197 | gga-miR-6606-5p, novel_miR_815 | MSTRG.33345.5, MSTRG.140060.1, MSTRG.18943.13 | Calcium ion transport |
| NGFR | 1.5519 | 0.0036 | novel_miR_676 | MSTRG.118362.24, MSTRG.12318.1, MSTRG.25780.4, MSTRG.128839.3, MSTRG.115756.46, MSTRG.41646.22, MSTRG.80622.27, MSTRG.44286.2, MSTRG.41646.23 | Blood vessel morphogenesis, blood vessel development, vasculature development, angiogenesis |
| ADAM8 | −1.3727 | 0.0470 | Response to hypoxia, blood vessel development, vasculature development, angiogenesis | ||
| CASQ2 | −1.0736 | 0.0315 | novel_miR_589, novel_miR_676 | MSTRG.73129.28, MSTRG.18943.3 | Blood circulation |
| IRF4 | −1.8880 | 0.0201 | Hemopoiesis | ||
| PTPRZ1 | 1.3653 | 0.0000 | Hemopoiesis, hematopoietic progenitor cell differentiation | ||
| CALML3 | −1.1958 | 0.0015 | Vascular smooth muscle contraction | ||
| ERBB4 | −1.2949 | 0.0102 | novel_miR_676, novel_miR_587 | MSTRG.147643.141, MSTRG.128534.3, MSTRG.44329.1, MSTRG.29590.15, MSTRG.79818.8, MSTRG.135465.5, MSTRG.30620.3 | Calcium and MAPK signaling pathways |
| ARR3 | 1.0513 | 0.0013 | MAPK signaling pathway | ||
| NTSR1 | −1.5401 | 0.0140 | novel_miR_669, novel_miR_676, novel_miR_567 | MSTRG.63187.27, MSTRG.71117.21, MSTRG.133793.2, MSTRG.29590.15, MSTRG.52146.15, MSTRG.72741.32, MSTRG.148631.1 | Regulation of respiratory gaseous exchange, Calcium signaling pathway |
| Energy metabolism | |||||
| SSTR5 | −3.8881 | 0.0054 | novel_miR_819 | MSTRG.25881.3 | Glucose homeostasis |
| NR1H4 | −1.1224 | 0.0179 | novel_miR_676 | MSTRG.99917.37, MSTRG.64717.15, MSTRG.99917.39, MSTRG.99917.35 | Glucose homeostasis, response to oxygen-containing compound |
| HTR2C | −1.3476 | 0.0000 | Regulation of lipid metabolic process | ||
| APOA1 | 1.1034 | 0.0068 | Regulation of lipid metabolic process | ||
| KCNB1 | −1.1800 | 0.0000 | Glucose homeostasis, response to oxygen-containing compound | ||
| P3H2 | −1.0044 | 0.0135 | Carbohydrate binding | ||
| CHST8 | −1.9553 | 0.0013 | novel_miR_693, novel_miR_775 | Carbohydrate metabolic process | |
| LYZ | −1.0365 | 0.0050 | Carbohydrate metabolic process | ||
| HAO2 | −1.5483 | 0.0085 | novel_miR_669 | Carbon metabolism, lactate oxidation | |
| ACER1 | 1.5111 | 0.0032 | Regulation of lipid metabolic process | ||
| ACSBG1 | 1.1154 | 0.0000 | Fatty acid metabolism | ||
| ELOVL2 | −5.1386 | 0.0480 | Fatty acid metabolism | ||
| ELOVL3 | 2.0872 | 0.0146 | novel_miR_867 | Fatty acid metabolism | |
| GBE | −1.0082 | 0.0072 | novel_miR_85 | MSTRG.128588.4 | Oxygen transport, oxygen binding, reactive oxygen species metabolic process |
| NOX3 | 3.0828 | 0.0000 | Reactive oxygen species metabolic process | ||