Fig. 4.

Dap treatment reduced the protein levels of SGK1, p‐Foxo1, and IL‐23R in kidney Th17 cells of db/db mice. Mice were randomly allocated into the following groups: Th17 cells were isolated from the kidneys in nondiabetic C57BLKS/J mice (NC), and diabetic db/db mice treated with normal saline (DM), Dap (1 mg·kg⁻¹), or voglibose (Vog; 0.6 mg·kg⁻¹) (n = 6 for each group). The Th17 cells were collected from CD4+ CD62L+ naive T cells after stimulation with recombinant TGF‐β (3 ng·mL⁻¹), IL‐6 (300 IU·mL⁻¹), and IL‐23 (300 IU·mL⁻¹). Total cell proteins were extracted from Th17 cells for western blot analyses. Equal amounts of protein (20 μg) extracted from Th17 cells were incubated with the following primary antibodies: SGK1, p‐Foxo1, and IL‐23R. (A) representative images for SGK1, p‐Foxo1, and IL‐23R expression; (B) semi‐quantitative analysis for SGK1 expression; (C) semi‐quantitative analysis for p‐Foxo1 expression; (D) semi‐quantitative analysis for IL‐23R expression. Quantitative data are presented as mean ± SD. One‐way ANOVA with SNK test or Dunnett's T3 test was used for statistical analysis. **, P < 0.01 compared with the NC group; ##, P < 0.01 compared with the DM group.