Fig. 6.

PI3K‐Akt pathway‐dependent regulation of CD133 in response to serum deprivation. (A) Immunoblot analysis. HCT116 cells were cultured in the presence of 1% fetal bovine serum for 3 days and then treated with PI3K inhibitor (LY294002) at 25 nm for 24 h. After treatment, cell lysates (30 µg per lane) were prepared and analyzed by immunoblotting with the indicated antibodies. Actin was used as a loading control. (B) Relative band intensities of the phosphorylated proteins standardized with respect to total proteins were determined based on three independent experiments. Data represent the mean ± SD (n = 3) and the asterisk indicates a statistically significant difference (P < 0.05, t‐test).