TABLE 3.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| WM3064 | Donor strain for conjugation; ΔdapA | W. Metcalf, UIUC |
| DH5α | Host strain for routine cloning | Lab stock |
| Pseudoalteromonas | ||
| R3 | Wild type | 36 |
| ΔsspA mutant | Mutant of strain R3 with disrupted gene sspA | 35 |
| ΔsspB mutant | Mutant of strain R3 with disrupted gene sspB | This study |
| ΔpigKLM mutant | Mutant of strain R3 with disrupted genes pigK, pigL, and pigM | This study |
| ΔpvdA mutant | Mutant of strain R3 with disrupted gene pvdA | This study |
| Δfur mutant | Mutant of strain R3 with disrupted gene fur | This study |
| Δhns mutant | Mutant of strain R3 with disrupted gene hns | This study |
| ΔrpoS mutant | Mutant of strain R3 with disrupted gene rpoS | This study |
| T1lg65 | Yellow-pigmented isolate | 67 |
| S. oneidensis MR-1 | Putrebactin-producing bacterium | 49 |
| Plasmids | ||
| pHGM01 | Apr Gmr Cmr; suicide vector | 62 |
| pBBR1MCS-5 | Gmr; delivery vector for gene complementation | 63 |
| pBBR1MCS-5-fur | Complement vector carrying fur | This study |
| pBBR1MCS-5-sspA | Complement vector carrying sspA | This study |