TABLE 4.

Primers used in this study

Primer	Primer sequence
In-frame deletion
pigKLM-5O	GGGGACAAGTTTGTACAAAAAAGCAGGCTTTGTCCAGCAGCATAGTGTT
pigKLM-5I	AATAATGCGCTGCAAGGGCCAGCGTACTGGTTCAACATGA
pigKLM-3O	GGGGACCACTTTGTACAAGAAAGCTGGGTCTGCAAACTAAATCCGACGC
pigKLM-3I	GGCCCTTGCAGCGCATTATTCGAATGCTTTGACCGAATCC
sspB-5O	GGGGACAAGTTTGTACAAAAAAGCAGGCTGAATGATCGAGCAGCCAGTC
sspB-5I	GGTCCGGGTTCGCTATCTAT CGAATCGGATGGTGACAGAGC
sspB-3O	GGGGACCACTTTGTACAAGAAAGCTGGGTTGTCAAAAGAGCCCCACGTAT
sspB-3I	ATAGATAGCGAACCCGGACCTGTCGGTTCAACAGCTCAACTC
hns-5O	GGGGACAAGTTTGTACAAAAAAGCAGGCTTTGCTTTAAGCCTGCGGAAT
hns-5I	GGTCCGGGTTCGCTATCTATGCATTCGTTGGTGTTAACTTAGAA
hns-3O	GGGGACCACTTTGTACAAGAAAGCTGGGTCTGAACCTGAGCGTGTCTGT
hns-3I	ATAGATAGCGAACCCGGACCGCGCTTTATTCAAATCACTGTAAC
rpoS-5O	GGGGACAAGTTTGTACAAAAAAGCAGGCTGCGTAAGATGGCGTTACTCAG
rpoS-5I	GGTCCGGGTTCGCTATCTATTACAACATGAGGGCTTGAGCA
rpoS-3O	GGGGACCACTTTGTACAAGAAAGCTGGGTATAAGGTACGATGGCGCTGG
rpoS-3I	ATAGATAGCGAACCCGGACCTTGGGCTCCTCGACTGAAAC
fur-5O	GGGGACAAGTTTGTACAAAAAAGCAGGCTAGTGGGCCTGGACTTAAACG
fur-5I	GTAGATCACCGCTAACGCAGCAGCGACGTAGAAGCGTGT
fur-3O	GGGGACCACTTTGTACAAGAAAGCTGGGTGGTACTACGGTGAGGCACAG
fur-3I	CTGCGTTAGCGGTGATCTACGTTGTGATCAGTCATTAATC
pvdA-5O	GGGGACAAGTTTGTACAAAAAAGCAGGCTACCCCACGACCATATAAGCG
pvdA-5I	GTAGATCACCGCTAACGCAGGGCCAAACCCGATCCCAAT
pvdA-3O	GGGGACCACTTTGTACAAGAAAGCTGGGTGTCGCGCAGCATATTGAGTT
pvdA-3I	CTGCGTTAGCGGTGATCTACGAGCAAACCCATGGCATCAG
qRT-PCR
fur-RT-F	TCGGTCTTGCAACGGTTTAC
fur-RT-R	TCTTCCTGGCGCGTTTCAAT
pvdA-RT-F	GCGTACGTCTGACCAAAAACC
pvdA-RT-R	AAATCTCAGCACCACTTTGCC
pigA-RT-F	TGTCTTTGTTCCCGAGTCGC
pigA-RT-R	TGCCAAATTGCTTACGTTCCT
16S-RT-F	CTGGAACTGAGACACGGTCC
16S-RT-R	GGAGTTAGCCGGTGCTTCTT
Complementation
sspA-CF	CCAAGCTTGGAGTAGAAGAGCACGGCGTAAA
sspA-CR	CGGAATTCCGACATTCAGCACAATCTGCCC
fur-CF	CCGGAATTCTGAGCTATATCAGGCATTGA
fur-CR	CGCGGATCCGCGGACTCTTCAAATTGCGT