FIG 2.

Phylogeographic analysis showing diversification of PD-causing isolates within the contiguous United States. Color represents isolates from the same geographical location: California (red), Texas (pink), Georgia (green), North Carolina (dark green), and Florida (yellow). Coordinates were recorded during field sampling. In the absence of this information, coordinates referring to the city or vineyard closest to the sample site were used. Florida coordinates were not available; the location shown on the map represents central Florida. Isolates from Southern and Northern California are shown within pale red circles. PD-causing strains were divided into three phylogenetically supported clades: PD-I (Southeast United States isolates exclusively), PD-II (Southern California and Texas isolates), and PD-III (both Southern and Northern California isolates and three Georgia isolates). The tree was built using the core genome alignment without removing recombinant segments. Bootstrap values mark branch support. The map was generated using the open-source R package phytools (https://www.rdocumentation.org/packages/phytools/versions/0.7-70).