FIG 4.
(a) Reversed-phase high-performance liquid chromatography (RP-HPLC) chromatograms. An overlay of the chromatograms at 225 nm (i), 300 nm (ii), 350 nm (iii), and 400 nm (iv) of the final purification step of the wild-type YL-1, the ΔpvdS mutant, and the ΔpvdS(pUCP26-pvdS) complemented strain, using a C16 column (4.6 mm by 250 mm), is shown. Here, 50% methanol was used as a negative control. The gradient of the acetonitrile-water mobile phase was from 50% to 0% acetonitrile over 10 min at a flow rate of 1 ml/min. (b) Fractions of strain YL-1 per 20 s under UV light. (c) Anti-Xoo assay of fractions in 1/2 TSA medium. The diameter of the inhibitory zones was measured when Xoo covered the entire control plates. Fifty percent methanol was used as the control.