TABLE 3.
Strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Relevant characteristicsa,b | Source or reference (yr) |
|---|---|---|
| Strains | ||
| Pseudomonas chlororaphis | ||
| YL-1 | Wild-type strain, Ampr | Liu et al. (2015) (21) |
| YL-1 ΔpvdS | pvdS in-frame deletion mutant of strain YL-1, Ampr | Liu et al. (2019) (48) |
| YL-1 ΔpvdL | pvdL in-frame deletion mutant of strain YL-1, Ampr | This study |
| YL-1 ΔpvdF | pvdF in-frame deletion mutant of strain YL-1, Ampr | This study |
| YL-1 ΔpvdS(pUCP26) | Mutant strain ΔpvdS harboring plasmid pUCP | This study |
| YL-1 ΔpvdS(pUCP26-pvdS) | Mutant strain ΔpvdS harboring plasmid pUCP-pvdS | This study |
| Escherichia coli | ||
| DH5α | F− φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK− mK+) phoA supE44 λ− thi-1 gyrA96 | Sangon Corp. |
| S17-1λpir | (F−)RP4-2-Tc::Mu aphA::Tn7 recA λpir lysogen Smr Tpr | Simon et al. (1983) (47) |
| JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB) [F′ traD36 proAB+ lacIq lacZΔM15] | Promega Corp. |
| Plasmids | ||
| pUCP26 | Broad-host-range vector; Tcr | West et al. (1994) (49) |
| pEX18Gm | Suicide plasmid with a sacB gene, Gmr | Hoang et al. (1998) (46) |
| pEX18-pvdS | pEX18GM with two flanking fragments of pvdS | Liu et al. (2019) (48) |
| pUCP26-pvdS | Recombinant plasmid | This study |
| pEX18-pvdL | pEX18GM with two flanking fragments of pvdL | This study |
| pEX18-pvdF | pEX18GM with two flanking fragments of pvdF | This study |
| Primers | ||
| pvdS-F1 | 5′-GGGGTACCACTGCTGTGGCGGGATGTTC (KpnI) -3′ | Liu et al. (2019) (48) |
| pvdS-R1 | 5′-CCCAAGCTTCCGCGATTTTGACCAGGATC (HindIII) -3′ | Liu et al. (2019) (48) |
| pvdS-F2 | 5′-CCCAAGCTTCACCTACGCCCGCCACTGAT (HindIII) -3′ | Liu et al. (2019) (48) |
| pvdS-R2 | 5′-GCTCTAGAGGATGGCGTGAGGAGTGGAT (XbaI) -3′ | Liu et al. (2019) (48) |
| pvdL-F1 | 5′-CGGAATTCGGAAGAAAATCGGCACCACG (EcoRI) -3′ | This study |
| pvdL-R1 | 5′-CGGGGTACCGAGGAACTGGCGCAGTACAT (KpnI) -3′ | This study |
| pvdL-F2 | 5′-CGGGGTACCCTCTGATCCTGCTCTTCGGC (KpnI) -3′ | This study |
| pvdL-R2 | 5′-GCTCTAGAGCCTGCTTGCGATAGTGGTC (XbaI) -3′ | This study |
| pvdF-F1 | 5′-CGGGGTACCTCGCCCAGAAAACCACCTTG (KpnI) -3′ | This study |
| pvdF-R1 | 5′-CCCAAGCTTCGGAAGCGGGTTACATCACC (HindIII) -3′ | This study |
| pvdF-F2 | 5′-CCCAAGCTTCTTTGCCGCTGAGGGATTGG (HindIII) -3′ | This study |
| pvdF-R2 | 5′-CGGAATTCGGAAAACGCCGCAGGAACTC (EcoRI) -3′ | This study |
| PvdS FP | 5′- CAGGAATTCATGACGGAACAAGTATCCAC (EcoRI) -3′ | This study |
| PvdS RP | 5′- CACAAGCTTTCAGTGGCGGGCGTAGGTGT (HindIII) -3′ | This study |
| RT-pvdS-F | 5′-GCGAGATGCCGTTGATCAGC-3′ | This study |
| RT-pvdS-R | 5′-TACGAGCAGTTCACCATCCG-3′ | This study |
| RT-16S rRNA gene-F | 5′-ACGTCCTACGGGAGAAAGC-3′ | This study |
| RT-16S rRNA gene-R | 5′-CGTGTCTCAGTTCCAGTGTGA-3′ | This study |
a
Ampr , Gmr, Tcr, resistance to ampicillin, gentamicin, and tetracycline, respectively.
b
Restriction enzyme digestion sites in sequences are bold.