TABLE 3.

List of strains used in this study

Strain	Organism	Characteristics	Reference
M145	S. coelicolor	Devoid of SCP1 and SCP2 plasmids	(29)
O4121	S. coelicolor	sco4121 cloned into pIJ86 under the strong constitutive promoter pErmE*	This work
O4122	S. coelicolor	sco4122 cloned into pIJ86 under the strong constitutive promoter pErmE*	This work
Δ4122	S. coelicolor	sco4122 knockout mediated by homologous recombination using the temp sensitive plasmid pKC1139	This work
Δ4121	S. coelicolor	sco4121 knockout mediated by homologous recombination using the temp sensitive plasmid pKC1139	This work
cΔ4122	S. coelicolor	Δ4122 complemented with native copies of sco4122 in the multicopy plasmid pIJ86 under pErmE* promoter	This work
pFpV27::luc	M. smegmatis	Modified Mycobacterium smegmatis cells with the luciferase reporter gene replacing the gfp reporter	This work
MS4122	M. smegmatis	Modified Mycobacterium smegmatis cells bearing sco4122 integrated into the genome	This work
P4121	M. smegmatis	P4121::luc transformed into WT M. smegmatis; cells not expressing SCO4122	This work
MS4122::P4121	M. smegmatis	MS4122 cells transformed with luciferase reporter construct with an upstream sco4121 promoter	This work
MS4122::Pmyc	M. smegmatis	MS4122 cells transformed with luciferase reporter construct with an upstream Pmyc promoter	This work
WTki	M. smegmatis	Empty plasmid pST-Ki integrated into WT M. smegmatis genome	This work
OE4121	E. coli	sco4121 cloned under the expression plasmid pET28a and transformed into E. coli BL21(DE3)	This work