TABLE 4.
List of primers used in this studya
| Primer name | Primer sequence | Reference |
|---|---|---|
| Primers for cloning sco4121 into S. coelicolor | ||
| O4121 Fp | TTAGAAGCTTGCGGACGATCCGAGAGATCA | This work |
| O4121 Rp | AATTAGATCTCGTCCTGCAGAAGCCCTGAGC | This work |
| Primers for cloning sco4122 into S. coelicolor | ||
| O4122 Fp | AATT GGATCCGGAGTTGCGTGCCATACTGC | This work |
| O4122 Rp | AATTAAGCTTCGCAGAGGGCTGTCAAGC | This work |
| Primers for deletion of sco4122 from S. coelicolor genome | ||
| Δ4122 LFR Fp | ATCAGAATTCGACAGAACCCAGTCCCCGTA | This work |
| Δ4122 LFR Rp | AATATCTAGAGGCGATCTGCTCTTCGAGT | This work |
| Δ4122 RFR Fp | ATATAAGCTTACTTGGGGGTCGTGCTGGTG | This work |
| Δ4122 RFR Rp | AATTTCTAGAGCGGAGGGGCGCGAGAAGT | This work |
| Primers for deletion of sco4121 from S. coelicolor genome | ||
| Δ4121 LFR Fp | AATCGAATTCTACGTCTATGTGGCGCAGGT | This work |
| Δ4121 LFR Rp | AGATGGATCCGAACAGCAGCGTGAAGGAC | This work |
| Δ4121 RFR Fp | AACGTCTAGAAGCTCACCGATGTCCAGAAT | This work |
| Δ4121 RFR Rp | ATCGAAGCTTCGAACTGGTCGCCAACTCC | This work |
| Primers for cloning sco4121 into E. coli BL21(DE3) cells | ||
| OE4121Fp | TGGCGCTAGCGCGGACGATCCGAGAGATCA | This work |
| OE4121Rp | CCGGAAGCTTCGTCCTGCAGAAGCCCTGAGC | This work |
a
Fp, forward primer; Rp, reverse primer. Boldface type indicates the sites for the restriction enzymes used.