TABLE 5.
Loci of heat resistance among meat-borne E. coli isolates
| Organisma | LHRb | Location | Coordinates | G+C content (%) | Length (bp)c | ORF no. | LHR variantd |
|---|---|---|---|---|---|---|---|
| Beef 873.10 | 873.10 LHR1 | Chromosome | 22754–51098 | 57.7 | 28,345 | 28 | ClpK2 |
| 873.10 LHR2 | Chromosome | 2186098–2207888 | 59.8 | 21,791 | 22 | ClpK2 | |
| 873.10p1 LHR | Plasmid | 35177–52973 | 62.3 | 17,797 | 17 | Hybrid | |
| 873.10p2 LHR | Plasmid | 65948–79550 | 61.7 | 13,603 | 15 | ClpK1 | |
| Sheep 2273PO3 | 2273PO3 LHR1 | Chromosome | 2941510–2921761 | 61.3 | 19,750 | 22 | ClpK2 |
| 2273PO3 LHR2 | Chromosome | 3923433–3938938 | 62.1 | 15,506 | 17 | ClpK1 | |
| 2273PO3p1 LHR | Plasmid | 55553–36612 | 61.4 | 18,942 | 20 | ClpK2 | |
| Beef 730V1 | 730V1 LHR | Chromosome | 3826448–3841903 | 62.2 | 15,456 | 17 | ClpK1 |
| Beef 97.3 | 97.3p1 LHR | Plasmid | 139116–124551 | 61.8 | 14,566 | 18 | ClpK1 |
a
All isolates possess evgAS (a global regulator of LHR) (13); ClpA, ClpB, and ClpX (Clp chaperones); and ClpP (Clp protease).
b
Loci of heat resistance were identified by mapping the genomes against previously characterized LHR regions.
c
Length of LHR regions between flanking mobile elements.
d
The LHR variant is determined based on its homology to one of the two known variants of LHR, ClpK1 LHR and ClpK2 LHR.