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. Author manuscript; available in PMC: 2021 May 3.
Published in final edited form as: J Allergy Clin Immunol. 2018 Aug 7;143(3):1119–1130.e3. doi: 10.1016/j.jaci.2018.06.046

Fig. 7. Blimp-1 inhibits differentiation of human Th9 cells.

Fig. 7.

(A) CD4+ T cells isolated from PBMCs of healthy donors and allergic asthmatic patients were differentiated under Th0- and Th9-polarizing conditions. After 4 days of culture, RNA was isolated, and (A) IL-9 and (B) Blimp-1 mRNA levels were determined using qPCR. Data (mean ± SD) are representative of six allergic asthmatic patients and the healthy donor. (C) Immunoblot analysis of Blimp-1 and β-actin in Th9 cells transduced with control lentivirus (Ctrl-LV) or Blimp-1-expressing lentivirus (Blimp-1-LV); the cells were from healthy donors. (D) IL-9 mRNA expression and (E) IL-9 levels in the culture supernatant of Th9 cells transduced with Ctrl-LV or Blimp-1-LV; the cells were from healthy donors (C1–4) or asthmatic patients (P1–4), and IL-9 mRNA expression and levels were determined by qPCR and ELISA, respectively. Data are representative of four asthmatic patients and four healthy donors.