Table 1.
Primer | Sequence (5′-3′) | Annealing temperature (°C) | References |
---|---|---|---|
Universal primers | F: TCCTACGGGAGGCAGCAGT | 60 | [20] |
R: GGACTACCAGGGTATCTAATCCTGTT | |||
Pg | F: ACCTTACCCGGGATTGAAATG | 58 | [20] |
R: CAACCATGCAGCACCTACATAGAA | |||
fimA I | F: CTGTGTGTTTATGGCAAACTTC | 58 | [21] |
R: AACCCCGCTCCCTGTATTCCGA | |||
fimA Ib | F: CAGCAGAGCCAAAAACAATCG | 58 | [14] |
R: TGTCAGATAATTAGCGTCTGC | |||
fimA II | F: GCATGATGGTACTCCTTTGA | 58 | [22] |
R: CTGACCAACGAGAACCCACT | |||
fimA III | F: ATTACACCTACACAGGTGAGGC | 58 | [21] |
R: AACCCCGCTCCCTGTATTCCGA | |||
fimA IV | F: CTATTCAGGTGCTATTACCCAA | 58 | [21] |
R: AACCCCGCTCCCTGTATTCCGA | |||
fimA V | F: AACAACAGTCTCCTTGACAGTG | 58 | [14] |
R: TATTGGGGGTCGAACGTTACTGTC | |||
Pg probe used in ISH | CAATACTCGTATCGCCCGTTATTC-Digoxin | [3] |
Pg: Porphyromonas gingivalis. ISH: In situ hybridization