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. 2021 May 3;21:228. doi: 10.1186/s12903-021-01580-6

Table 1.

Specific oligonucleotides used in this study

Primer Sequence (5′-3′) Annealing temperature (°C) References
Universal primers F: TCCTACGGGAGGCAGCAGT 60 [20]
R: GGACTACCAGGGTATCTAATCCTGTT
Pg F: ACCTTACCCGGGATTGAAATG 58 [20]
R: CAACCATGCAGCACCTACATAGAA
fimA I F: CTGTGTGTTTATGGCAAACTTC 58 [21]
R: AACCCCGCTCCCTGTATTCCGA
fimA Ib F: CAGCAGAGCCAAAAACAATCG 58 [14]
R: TGTCAGATAATTAGCGTCTGC
fimA II F: GCATGATGGTACTCCTTTGA 58 [22]
R: CTGACCAACGAGAACCCACT
fimA III F: ATTACACCTACACAGGTGAGGC 58 [21]
R: AACCCCGCTCCCTGTATTCCGA
fimA IV F: CTATTCAGGTGCTATTACCCAA 58 [21]
R: AACCCCGCTCCCTGTATTCCGA
fimA V F: AACAACAGTCTCCTTGACAGTG 58 [14]
R: TATTGGGGGTCGAACGTTACTGTC
Pg probe used in ISH CAATACTCGTATCGCCCGTTATTC-Digoxin [3]

Pg: Porphyromonas gingivalis. ISH: In situ hybridization