Fig. 5.

In vivo Matrigel plug assay. a A schematic diagram of the experimental flow of the in vivo Matrigel plug assay. b Representative macroscopic images of explanted Matrigel plugs (7 days after implantation). c H&E-stained sections of Matrigel plugs. d Immunofluorescence-stained sections of Matrigel plugs for anti-human vWF (red). Nuclei were counterstained with DAPI (blue). f Mouse CD31 (green) and human vWF (red) double staining showed the interaction of human SCAP-derived ECs and host cells in the newly generated blood vessels. All images are representative from 3 animals each group and 2 implants per mouse. Scale bar = 50 μm. ^*P < .05, ^**P < .01