Fig. 9.

Knockdown of TRPC7 decreased while overexpression of TRPC7 increased the phosphorylation of RyR2 and PLN, respectively, in NRVMs. Western blotting showing the expression of a total RyR2, b p(S2814)RyR2, c total PLN, and d p(T17) PLN in NRVMs infected with different adenoviruses to knockdown or overexpress TRPC7. e–h Bar charts showing the quantification of each protein from a–d. To eliminate the loading bias, intensity of each target protein was normalized to that of its corresponding β-tubulin. The change of TRPC7 expression did not alter the expression of total RyR2 and PLN. However, knockdown of TRPC7 decreased while overexpression of TRPC7 increased the phosphorylation form of RyR2 [p(S2814)RyR2] and PLN [p(T17)PLN]. Data were presented as mean ± SEM (n = 4). *P < 0.05, ***P < 0.001 vs corresponding controls