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. 2021 Apr 20;59(5):e02784-20. doi: 10.1128/JCM.02784-20

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FIG 2 — Genome coverage and sequence mapping alignment depth for MiSeq reads data. SARS-CoV-2 isolate R4717 RNA serial dilutions were subjected to Fluidigm RT-PCR amplification and MiSeq sequencing. Data were mapped using genome NC_045512.2 as the reference. (A) Genome coverage for 10× serial dilutions of R4717 RNA from 1.0E + 06 (top) to 1 (bottom) copies. Mapping depth was indicated in colors as follows: green, normal depth (depth ≥ 10); cyan, dip (depth 1 to 10); red, gap. Results for four replicates are shown. (B) Sequence mapping graphs for 10⁶, 10⁵, 10⁴ and 10³ copies of RNA from one set of serial dilutions.