TABLE 1.

PCR primers used to amplify select portions of H. pylori genes

Antibiotic	Gene	Primer name	Nucleotide sequence	Product size (bp)	Reference
Metronidazole (MTZ)	rdx	forward primer rdx1	5′-GCC ACT CCT TGA ACT TTA ATT TAG G-3′	749	(63)
		reverse primer rdx4	5′-CGT TAG GGA TTT TAT TGT ATG CTA C-3′
	frx	forward primer frx1	5′-CGA ATT GGA TAT GGC AGC CG-3′	913	(63)
		reverse primer frx4	5′-TAT GTG CAT ATC CCC TGT AGG -3′
Clarithromycin (CLR)	23S	forward primer 23SF2	5′-CGG TGC TCG AAG GTT AAG AG-3′	913	This study
		reverse primer 23S R2	5′-TTC AGC GGT TAT CAC ATC CA -3′
Levofloxacin (LEV)	gyrA	forward primer gyrA1	5′-TTT AGC TTA TTC AAT GAG CGT-3′	428	(64)
		reverse primer gyrA2	5′-GCA GAC GGC TTG GTA GAA TA -3′
Rifampin (RIF)	rpoB	forward primer1369f	5′-AGG GAC CAC TTG GGC AAT CGT AGG-3′	498	(65)
		reverse primer1867r	5′-TAG CGG TCA AAT AAA TCG TCT CAC -3′
Tetracycline (TET)	16S	forward primer-9F	5′-GAG TTT GAT YCT GGC TCA G-3′	1532	This study
		reverse primer-1541R	5′-AAG GAG GTG WTC CAR CC-3′
Amoxicillin (AMX)	pbp1	forward primer pbpF1	5′-TGC GAA CAC CCT TTT AAA T-3′	2385	(66)
		reverse primer pbpR1	5′-GCG ACA ATA AGA GTG GCA-3′