FIG 1.

Alignment of cps operons from the four known serogroup 15 serotypes and from the two isolates expressing the new serotype 15D. The three genes indicated in red provide basis by which to resolve the five different serotypes shown. The gray open reading frames encode enzymes that have functions unrelated to serogroup 15 polysaccharide structure. The short black open reading frames are transposase gene remnants. The two serotype 15D strains were subjected to whole-genome sequencing as previously described (1, 2). Sequences encompassing the dexB 3′ end and the aliA 5′ end were extracted from these two strains and were aligned with published GenBank sequences for cps15F (CR931666), cps15A (CR931663), cps15B (CR931664), and 15C (CR931665) (see references 4 and 6). The extracted sequences and published serogroup 15 cps operons were subjected to Prokka to identify and annotate open reading frames (9). The resultant annotated sequences were analyzed by BLAST and the homolog cps locus regions were aligned into figures using EasyFig version 2.2.3 software (10). Strains 84245 and MZ877 were assigned BioSample accession numbers SAMN14150919 and SAMN17684515, respectively, under project number PRJNA697931.