FIGURE 3.

PLP promotes PB protrusion in NC 10 embryos. (a) Single focal plane images of NC 10 embryos of the indicated genotypes stained with anti-Vas (green) to label germ plasm, anti-Asl (magenta) to label the centrioles, and phalloidin (red) to label the actin cortex. Bar, 5 μm. (b) Quantification of the distance between centrosomes and the proximal posterior cortex at NC 10. Each data point indicates the distance between one centrosome and the cortex in mCherry^RNAi (N = 66 events in 10 embryos) and plp^RNAi (N = 70 events in 12 embryos). Mean ± SD distances are displayed (red). n.s., not significant by Student’s t test. The experiment was performed twice with similar results. (c) Quantification of embryos with PB protrusion defects in NC 10. PBs were observed in 88% of mCherry^RNAi embryos versus 45% in plp^RNAi embryos (N = 33 embryos per genotype). NC, nuclear cycle; PB, pole bud; PLP, Pericentrin-like protein