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. Author manuscript; available in PMC: 2021 May 3.
Published in final edited form as: Methods Mol Biol. 2019;1999:173–184. doi: 10.1007/978-1-4939-9500-4_10

Figure 1.

Figure 1.

Crosslinked plasmid DNA was replicated for 60 minutes in HSS/NPE and then incubated with a range of formaldehyde concentrations in the Formaldehyde Crosslinking Buffer (see Step 3.3-1) prior to immunoprecipitation with anti-RPA antibodies [9]. Percent recovery of DNA near the crosslink is plotted for each formaldehyde concentration tested.