| Antibodies |
| Anti-TNF-α neutralizing antibody |
Cell Signaling Technology |
Cat# 7321S |
| Anti-C1q neutralizing antibody |
Quidel |
Cat# A301 |
| Anti-IL-1α neutralizing antibody |
abcam |
Cat# ab9614 |
|
Bandeiraea Simplicifolia Lectin I |
Vector Labs |
Cat# B-1105 |
| Donkey anti-Goat Alexa Fluor 647 secondary antibody |
Thermo Fisher |
Cat# A21447 |
| Donkey anti-Rabbit Alexa Fluor 488 secondary antibody |
Thermo Fisher |
Cat# A21206 |
| Goat ant-mouse IgM μ-chain |
Jackson ImmunoResearch |
Cat# 115-005-02 |
| Goat anti-mouse IgG+IgM (H+L) |
Jackson ImmunoResearch |
Cat# 115-005-044 |
| Goat anti-rat IgG (H+L) |
Jackson ImmunoResearch |
Cat# 112-005-167 |
| Goat polyclonal antibody against ChAT |
Sigma |
Cat# AB144P |
| Mouse anti-human Integrin beta 5 |
eBioscience |
Cat# 14-0497-82 |
| O4 hybridoma supernatant |
Sommer and Schachner, 1981 |
N/A |
| Rabbit polyclonal antibody against Lucifer Yellow |
Invitrogen |
Cat# A-5750 |
| Rabbit polyclonal antibody against RBPMS |
PhosphoSolutions |
Cat# 1830-RBPMS |
| Rat anti-mouse CD45 |
BD Pharmingen |
Cat# 550539 |
| Chemicals, Peptides, and Recombinant Proteins |
| Ames’ medium |
Sigma |
Cat# A1420 |
| Apo-transferrin |
Sigma |
Cat# T1147 |
| ATP-magnesium salt |
Sigma |
Cat# A9187 |
| Bovine serum albumin (BSA) |
Sigma |
Cat# A4161 |
| DMEM (high glucose) |
GIBCO |
Cat# 11960-044 |
| DNase I |
Worthington |
Cat# DPRFS |
| Donkey serum |
EMD Millipore |
Cat# S30-100ML |
| Earle’s balanced salt solution |
Sigma |
Cat# E7510 |
| EDTA-free Protease Inhibitor Cocktail |
Sigma |
Cat# 04693159001 |
| EGTA |
Sigma |
Cat# E3889 |
| Fetal calf serum (FCS) |
Gibco |
Cat# 10437-028 |
| FluoSpheres™ Polystyrene Microspheres, 10 μm, red fluorescent (580/605) |
Invitrogen |
Cat# F8834 |
| Goat serum |
MP Biomedicals |
Cat# 0219135680 |
| GTP-sodium salt |
Sigma |
Cat# G8877 |
| HEPES |
Sigma |
Cat# H3375 |
| L-cysteine hydrochloride monochloride |
Sigma |
Cat# C7880 |
| L-glutamine |
GIBCO |
Cat# 25030-081 |
| N-acetyl cysteine |
Sigma |
Cat# A9165 |
| Native hC1q |
Mybiosource |
Cat# MBS143105 |
| Neurobasal |
GIBCO |
Cat# 21103-049 |
| Ovomucoid trypsin inhibitor |
Worthington |
Cat# LS003086
|
| Papain |
Worthington |
Cat# LS003126
|
| Paraformaldehyde Aqueous Solution |
Electron Microscopy Sciences |
Cat# 15710 |
| Penicillin/ Streptomycin |
GIBCO |
Cat# 15140-122 |
| Poly-D-lysine hydrobromide |
Sigma |
Cat# P6407 |
| Potassium methanesulfonate |
Sigma |
Cat# 83000 |
| Progesterone |
Sigma |
Cat# P-8783 |
| Putrescine |
Sigma |
Cat# P-5780 |
| Recombinant HB-EGF |
PeproTech |
Cat# 100-47 |
| Recombinant hTNFa |
Cell Signaling Technology |
Cat# 8902 |
| Recombinant II-1a |
Sigma |
Cat# I3901 |
| Sodium chloride |
Sigma |
Cat# S9888 |
| Sodium pyruvate |
Sigma |
Cat# P5280 |
| Sodium selenite |
Sigma |
Cat# S-5261 |
| SYBR Green PCR Master Mix |
Thermo Fisher |
Cat# 4385612 |
| TEA-Cl |
Sigma |
Cat# T2265 |
| Triton X-100 |
Sigma |
Cat# T8787 |
| Trypsin |
Sigma |
Cat# T9935 |
| Critical Commercial Assays |
| 96.96 Dynamic Array™ IFC for Gene Expression (includes Control Line Fluid, 2x Assay Loading Reagent, 20x GE Sample Loading Reagent, PreAmp Master Mix, RT Master Mix) |
Fluidigm |
Cat# 101-0349 |
| Bradford Assay |
Bio-Rad |
Cat# 500-0006 |
| High-Capacity RNA-to-cDNA Kit |
Thermo Fisher Scientific |
Cat# 4387406 |
| RNeasy Micro Kit |
QIAGEN |
Cat# 74004 |
| Experimental Models: Organisms/Strains |
| Mouse: C57Bl/6J |
Jackson Laboratory |
Strain Code 000664 |
| Mouse: II1a-/-Tnf-/-C1qa-/-
|
Liddelow et al., 2017 |
N/A |
| Rat: Sprague Dawley |
Charles River |
Strain Code 400 |
| Software and Algorithms |
| FIJI/ImageJ |
NIH |
N/A |
| MATLAB |
MathWorks |
N/A |
| Psychophysics Toolbox |
|
https://github.com/kleinerm/Psychtoolbox-3 |
| Fluidigm Melting Curve Analysis Software 1.1.0 build 20100514.1234 |
Fluidigm |
N/A |
| BioMark Data Collection Software 2.1.1 build 20090519.0926 |
Fluidigm |
N/A |
| Real-time PCR Analysis Software 2.1.1 build 20090521.1135 |
Fluidigm |
N/A |
| pClamp 11.0 |
Molecular Devices |
N/A |
| Other |
| 1.0 Neutral Density Filter |
Chroma |
Cat# ND 1.0 |
| 30 kDa MWCO centrifugal filter unit |
Sartorius |
Cat# VS2022 |
| 5μl Hamilton Syringe |
Hamilton Company |
Cat# 87919 |
| DLP® LightCrafter™ E4500 MKII™ Fiber Couple |
EKB Technologies |
Cat# E4500MKII |
| icare® TONOLOAB rebound tonometer |
icare |
Cat# TV02 |
| IR (830 nm) LED Spotlight |
Edmunds Optics |
Cat# 66-860 |
| Picospritzer III - Intracellular Microinjection Dispense Systems |
Parker Precision Fluidics |
Cat# 051-0500-900 |
| Poly-D-Lysine coated coverslips (12 mm) |
Neuvitro |
Cat# NC0565504 |
| Polystyrene Microspheres, 10 μm |
Invitrogen |
Cat# F-8834 |
| UV DLP Projector |
EKB Technologies |
Cat# DPM-E4500UVGOAMKII |
