| 200× Hepes | Final concentration | Amount |
|---|---|---|
| Hepes | 1 M | 119.15 g |
| Milli-Q water | To a final volume of 500 mL | |
| Total | 1 M | 500 mL |
Adjust pH to 7.4 with NaOH. Can be stored at +4°C for several months.
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