Table 1.
Experimental infections of Galba truncatula lymnaeid snails with Fasciola hepatica pig isolate both from the Northern Bolivian Altiplano human hyperendemic area, and their comparison with results obtained in the F. hepatica sheep and cattle isolates from the same endemic area.
| Host isolate |
Pig |
Sheepa |
Cattlea |
||||||
|---|---|---|---|---|---|---|---|---|---|
| F. hepatica geogr. Origin | Batallas | Batallas | Batallas | Batallas | Batallas | Batallas | Batallas | Kallutaca | Kallutaca |
| Lymnaeid geogr. Origin | Huacullani | Huacullani | Ancocagua | Huacullani | Huacullani | Ancocagua | Huacullani | Viacha | Tambillo |
| Miracidial dose | mono-miracidial | mono-miracidial | tri-miracidial | mono-miracidial | mono-miracidial | bi-miracidial | mono-miracidial | mono-miracidial | tri-miracidial |
| Temperature (12 h day/12 h night) | 20° C/20 °C | 22 °C/5 °C | 20 °C/20 °C | 20 °C/20 °C | 22 °C/5 °C | 20° C/20 °C | 20 °C/20 °C | 22 °C/5 °C | 20 °C/20 °C |
| No. lymnaeids infected | 92 | 46 | 79 | 62 | 50 | 37 | 55 | 21 | 50 |
| No. survivor snails at beginning of shedding (%) | 54 (58.7%) | 43 (93.5%) | 24 (30.4%) | 54 (87.1%) | 41 (82.0%) | 13 (35.1%) | 48 (87.3%) | 13 (61.9%) | 23 (46.0%) |
| No. shedding snails (%) | 16 (29.6%) | 5 (13.7%) | 4 (16.7%) | 28 (51.8%) | 9 (21.9%) | 7 (53.8%) | 12 (25.0%) | 2 (15.4%) | 15 (65.2%) |
| Prepatent period in dpi (mean) | 49–55 (51.1) | 48–59 (53.0) | 44–57 (53.5) | 48–92 (55.6) | 59–93 (69.3) | 41–69 (49.7) | 49–76 (55.5) | 94–95 (94.5) | 43–95 (67.2) |
| Shedding end in dpi (mean) | 69–131 (119.1) | 48–62 (56.6) | 49–71 (59.5) | 52–136 (89.4) | 70–93 (75.7) | 49–87 (67.6) | 58–135 (101.6) | 95–106 (100.5) | 70–117 (89.3) |
| Shedding length in days | 20–83 (70.1) | 1–11 (4.6) | 1–15 (6.7) | 1–88 (34.7) | 1–15 (7.3) | 6–29 (18.9) | 1–85 (47.1) | 1–13 (7.0) | 1–85 (23.1) |
| No. total cercariae shed | 3859 | 204 | 130 | 5542 | 311 | 429 | 3672 | 30 | 762 |
| No. cercariae/snail (mean) | 101–569 (428.8) | 1–131 (40.8) | 19–47 (32.5) | 8–562 (197.9) | 1–155 (34.5) | 15–101 (61.3) | 8–581 (306.0) | 1–29 (15.0) | 2–100 (51.8) |
| Snail survival after shedding end in days | 1–73 (38.7) | 0–83 (21.8) | 0–1 (0.75) | 1–132 (24.5) | 0–34 (5.0) | 1–10 (3.9) | 1–133 (42.3) | 0–25 (12.5) | 1–21 (3.8) |
| Longevity of shedding snails in dpi | 92–181 (157.8) | 59–138 (78.4) | 50–72 (60.2) | 53–192 (113.8) | 72–127 (75.9) | 51–90 (71.4) | 76–268 (143.9) | 106–120 (113.0) | 71–128 (93.1) |
| Longevity of non-shedding snails in dpi | 98–180 (140.3) | 48–153 (98.3) | 31–62 (43.5) | 49–196 (139.1) | 56–143 (83.2) | 44–107 (62.7) | 31–209 (105.4) | 96–161 (132.9) | 32–49 (38.5) |
dpi = days post-infection.
a
Data from Mas-Coma et al. [36].