Table 2.

LC gradient condition for untargeted analysis

Time (min)	Gradient (% B)	Flow rate (mL/min)
0.0	0	0.3
1.0	0	0.3
5.0	40	0.3
7.5	64	0.3
12.0	64	0.3
12.5	82.5	0.3
19.0	85	0.3
20.0	95	0.3
20.1	0	0.3
25.0	0	0.3