Reagent	Final concentration	Amount
10 mg/L Prostaglandin B2-d4 methanol solution	295 μM (100 μg/L)	10 μL
10 mg/L 8-iso Prostaglandin F2α-d4 methanol solution	279 μM (100 μg/L)	10 μL
Methanol for LCMS	n/a	980 μL
Total	n/a	To 1 mL