Fig. 3.

Vascular leakage of 2 × 10⁶ Da rhodamine-labeled dextran. Brain, skeletal muscle and distal colon were prepared and evaluated as described in materials and methods. Values reflect fluorescence from rhodamine as arbitrary units/mg tissue protein in homogenates from mice subjected to 2 h of air or air plus 1,000 to 10,000 ppm CO₂. Where shown, mice were injected with PEG, nonspecific control IgG, anti-IL-1β IgG, or anakinra just before exposure to 4,000 ppm CO₂. Others were injected with Ly6G-positive or -negative MPs prepared from mice exposed to 4,000 ppm CO₂ and killed 1 h later. Data are means ± SE. *P < 0.05, significantly different from control by ANOVA.