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. 2021 May 1;35(9-10):729–748. doi: 10.1101/gad.346791.120

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© 2021 Ito et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Brown adipose tissue is formed in Myf5^CreMed1^f/f mice. (A) Macroscopic examination of BAT of Myf5^CreMed1^f/w and Myf5^CreMed1^f/f mice at perinatal (E17.5 and P1) stages. (Left panel) White arrows indicate the Myf5^CreMed1^f/f mice. BAT is present at the upper back of the Myf5^CreMed1^f/f mice. (B) H&E staining of thoracic transversal cryosection of E17.5 Myf5^CreMed1^f/w and Myf5^CreMed1^f/f mice. Arrowheads indicate the location of BAT. (C) Percentage of BAT weight over body weight of Myf5^CreMed1^f/w (n = 4) and Myf5^CreMed1^f/f (n = 5) mice at P1 (mean ± SD, unpaired t-test, P < 0.005). (D) Immunohistochemistry of MED1 in E17.5 BAT regions. Absence of MED1 in Myf5^CreMed1^f/f mice is shown. Scale bars, 100 μm. (E) Immunohistochemistry of PPARγ (BAT marker) and MyHC (skeletal muscle) in E17.5 embryos. Scale bars, 100 μm.