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. 2021 May 1;35(9-10):698–712. doi: 10.1101/gad.348431.121

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© 2021 Viktorovskaya et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 1. — Mutations in SPT16/POB3 and SPT5 suppress spt6-YW in an allele-specific fashion. (A) Western blots showing the levels of Spn1, Rpb1, and Spt6-FLAG in Spt6-FLAG immunoprecipitation (IP) samples and the corresponding inputs from untagged control (FY87), wild-type (FY3276), spt6-YW (FY3277), and spt6-1004 (FY3283) strains. Spn1, Rpb1, and Spt6 were detected using anti-Spn1, 8WG16 anti-Rpb1, and anti-FLAG antibodies, respectively (Supplemental Table S5). (B) A schematic showing the isolation of spt6-YW suppressors, with genes identified as extragenic suppressors listed at the right. (C,D) Analysis of genetic interactions between spt6, spt5, pob3, and spt16 mutations. Strains were grown to saturation in YPD, serially diluted 10-fold, spotted on the indicated media, and grown at the indicated temperature.