Skip to main content
. 2021 May 1;35(9-10):698–712. doi: 10.1101/gad.348431.121

Figure 2.

Figure 2.

Spt6, FACT, Spt5, and Spn1 functionally interact to modulate nucleosome organization in vivo. (A) Average MNase-seq dyad signal over 3086 nonoverlapping verified coding genes aligned by the 30°C wild-type +1 nucleosome dyad, for wild-type (FY87), spt6-YW (FY3223), pob3-E154K (FY3206), spt6-YW pob3-E154K (FY3205), spt5-QS (FY3273), and spt6-YW spt5-QS (FY3274) strains grown at 30°C or with a shift to 37°C. Values are the mean of the mean library-size normalized coverage over the genes considered, over at least two replicates. (B) Mean differences in nucleosome position between mutant and wild type, quantified from MNase-seq data. Nucleosomes are grouped based on their position in the nucleosome array of a gene, with the +1 nucleosome defined as the nucleosome region with midpoint position immediately 3′ of the TSS. (C) Distributions of nucleosome fuzziness, defined as the standard deviation of MNase-seq dyad positions within a nucleosome region. Vertical lines indicate median values of each distribution.