Figure 3.

Gene annotation reveals previously unknown genes in the genome of N. caninum. (A) Graphical representation of the position of novel genes in the newly assembled chromosomes. Red lines mark the position of novel genes along chromosomes. Alignment to the previously assembled NcLiv genome is partially shown for reference. Three insets are shown to highlight the annotation of three new genes in three newly assembled genomic regions. (B) Putative function of six out of the 38 newly identified genes. The remaining 32 genes are annotated as hypothetical. (C) Representative example of the improvement in annotation in regions that had been previously collapsed owing to the presence of tandem repeats. Several new genes were annotated, all of whose annotation is supported by RNA-seq data.