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. Author manuscript; available in PMC: 2021 May 3.
Published in final edited form as: Science. 2020 Feb 7;367(6478):681–684. doi: 10.1126/science.aay9847

Fig. 4: Ir21a promotes warmth-stimulated blood feeding.

Fig. 4:

(A-E) Host approach. Mosquitoes were activated by five breaths, then presented a hand. (B) Mosquitoes landed (accumulated) on cage roof below hand. Average +/− SEM. 33–75 females/assay. wild type, n=19 independent groups; Ir21aEYFP , n=21. (C) Average accumulation, 180s to 300s. (D) Accumulation rate=[{(accumulation at 45s) – (accumulation pre-hand exposure)}/{(maximum accumulation) – (accumulation pre-hand exposure)}]/45s. (E) Departure rate=[1 – {(accumulation at 340s)/(accumulation at 300s)}]/40s. (F-H) Blood-feeding. Two meals (one dyed green) placed on cage. (G) RT meal dyed. (H) Warm meal dyed. Dotted lines link pairs. 33-75 females/assay. n=6 independent groups/genotype, except H, Ir21aEYFP n=7. p values indicate t-test results. The green tracking dye partially reduced relative consumption of the meals to which it was added. Cages are 17.5 cm-sided cubes. Shapiro-Wilk test values are provided in statistics section of methods.