Figure 4. PMS2 and LIG4 are downregulated after acute and chronic hypoxic exposure.

(A) D566-MG cells were incubated in 1% O₂ for 1-5 days, as well as 21% O₂. After incubation, RNA was extracted and converted to cDNA for RT-PCR experiments, probing for LIG4 and PMS2. Data is represented as log₂ fold change in mRNA levels with respect to level for the 21% O₂ samples. Data are the mean of at least three independent experiments, with error bars showing S.E.M. * Denotes significant data where p < 0.05 indicated by one-way student t-test. (B) Western blot of PMS2 in D566-MG and U87-MG cells, incubated in 21%, 1% or 0.1% O₂ for 5 days. β actin was used as a loading control. Experiments were performed in triplicate, a representative blot is shown.